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human cyclin d1 rabbit polyclonal igg cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc human cyclin d1 rabbit polyclonal igg cell signaling technology
    Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand <t>cyclin</t> <t>D1/CD9</t> expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).
    Human Cyclin D1 Rabbit Polyclonal Igg Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 5319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cyclin d1 rabbit polyclonal igg cell signaling technology/product/Cell Signaling Technology Inc
    Average 98 stars, based on 5319 article reviews
    human cyclin d1 rabbit polyclonal igg cell signaling technology - by Bioz Stars, 2026-02
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    1) Product Images from "The effect of heat shock protein 27 on extravillous trophoblast differentiation and on eukaryotic translation initiation factor 4E expression."

    Article Title: The effect of heat shock protein 27 on extravillous trophoblast differentiation and on eukaryotic translation initiation factor 4E expression.

    Journal: Molecular human reproduction

    doi: 10.1093/molehr/gau002

    Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand cyclin D1/CD9 expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).
    Figure Legend Snippet: Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand cyclin D1/CD9 expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).

    Techniques Used: Migration, Control, Western Blot, Transfection, Wound Healing Assay, Activity Assay, Expressing



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    human cyclin d1 rabbit polyclonal igg cell signaling technology  (Cell Signaling Technology Inc)
    98
    Cell Signaling Technology Inc human cyclin d1 rabbit polyclonal igg cell signaling technology
    Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand <t>cyclin</t> <t>D1/CD9</t> expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).
    Human Cyclin D1 Rabbit Polyclonal Igg Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cyclin d1 rabbit polyclonal igg cell signaling technology/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    human cyclin d1 rabbit polyclonal igg cell signaling technology - by Bioz Stars, 2026-02
    98/100 stars
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    Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand cyclin D1/CD9 expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).

    Journal: Molecular human reproduction

    Article Title: The effect of heat shock protein 27 on extravillous trophoblast differentiation and on eukaryotic translation initiation factor 4E expression.

    doi: 10.1093/molehr/gau002

    Figure Lengend Snippet: Figure 4 The effect of HSP27 silencing on HTR-8/SVneo cell phenotype. The effect of HSP27 silencing on HTR-8/SVneo cell death, Sub-G1 phase, cell migration, MMPs activityand cyclin D1/CD9 expressionwasanalyzed. (A) HSP27 expressionin HTR-8/SVneo cells exposedto control/HSP27 siRNA for 72 and 96 h by western blot. (B) Cell death results obtained by Annexin-V/7AAD (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo. (C) Sub-G1 phase results obtained by PI (FACS) of the control/HSP27 siRNA transfected HTR-8/SVneo cells. (D and E) Cell migration of the control/HSP27 siRNA transfected HTR-8/SVneo cells wastested by the Scratch assay. (F) Zymogram assay analyses of MMP2/9 activity in media collected from control/ HSP27 siRNA transfected HTR-8/SVneo cells following 48, 72 and 96 h. (G) Western blot analyses of CD9 and cyclin D1 expression levels of HTR-8/ SVneo cells exposed to control/HSP27 siRNA. *Significantly different from control siRNA (P , 0.05, n ≥4).

    Article Snippet: The number of sectors in which EVT cells appeared was ............................................................................................................................................................................................. Target Source Isotype Company Dilutions Method Human HLA-G Mouse mAb IgG1 Acris 1:500 WB 1:200 IHC Human HSP27 Mouse mAb IgG1 Chemicon 1:200 WB 1:100 IHC Human Ki67 Mouse mAb IgG1 Zymed 1:150 IHC Human Caspase 3 (cleaved) Rabbit Polyclonal IgG Biocare medical 1:50 IHC Human E-cadherin Rabbit mAb IgG Cell Signaling Technology 1:1000 WB IgG Isotype control Rabbit Polyclonal IgG Dako 1:20000 IHC IgG1 Isotype control Mouse mAb IgG1 R&D Systems 1:2000 IHC Human total eIF4E Rabbit Polyclonal IgG Cell Signaling Technology 1:1000 WB 1:100 IHC Human Phospho-eIF4E (Ser209) Rabbit Polyclonal IgG Cell Signaling Technology 1:1000 WB Human total 4E-BP1 Rabbit Polyclonal IgG Cell Signaling Technology 1:1000 WB Human Phospho-4EBP1 (ser65) Rabbit mAb IgG Cell Signaling Technology 1:1000 WB Human total MNK1 Rabbit mAb IgG Cell Signaling Technology 1:1000 WB Human phospho-MNK1 (Thr197/202) Rabbit mAb IgG Epitomics 1:1000 WB Human Phospho-P53 (Ser 46) Rabbit Polyclonal IgG Cell Signaling Technology 1:1000 WB Human Cyclin D1 Rabbit Polyclonal IgG Cell Signaling Technology 1:1000 WB Human CD9 Mouse mAb IgG1 Acris 1:500 WB Human Tubulin Mouse mAb IgG1 Sigma 1:8000 WB Peroxidase conjugated anti-mouse Goat Polyclonal IgG Jackson Immuno-Research 1:40000 WB Peroxidase conjugated anti-Rabbit Goat Polyclonal IgG Jackson Immuno-Research 1:10000 WB SuperPicTure Polymer detection kit (HRP) Zymed Lab IHC D ow nloaded from https://academ ic.oup.com /m olehr/article/20/5/422/1214684 by Indian Institute of Technology Bom bay user on 16 June 2024 calculated [for example, nine sectors were considered as 75% effect (9/12 × 100 1⁄4 75%)].

    Techniques: Migration, Control, Western Blot, Transfection, Wound Healing Assay, Activity Assay, Expressing